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Low-dose arsenite at 0.1 μM increased mono- (A) and dimethylated (B) H3K9 but had no effect on trimethylated (C) H3K9 in A549 cells. Arsenite at higher doses (1, 5 or 10 μM) increased di- (B) and tri- (C) but not monomethylated (A) H3K9 in A549 cells. A549 cells were treated with different concentrations of arsenite for 24 h, followed by immunofluorescent staining with mono-, di- and trimethylated H3K9 antibodies as described in Materials and Methods.
