Fig. 4.

Inhibitory effect of GUG on TPA-induced phosphorylation of MAPKs, activation of NF-κB and IKKα/β, phosphorylation and degradation of IκBα in SENCAR mice. (A and B) At different time after treatment, the animals were killed, epidermal cytosolic and nuclear lysates were prepared and protein expression was determined as described in text. Equal loading was confirmed by stripping the immunoblot and reprobing it for β-actin. The immunoblots shown here are representative of three independent experiments with similar results. The values above the figures represent relative density of the band normalized to β-actin.