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. 2008 Aug 1;29(11):2175–2181. doi: 10.1093/carcin/bgn179

Fig. 3.

Fig. 3.

Effects of selenite, 5-Aza-dC and TSA on histone modifications, DNMT1 protein levels and interaction of histone or DNMT1 with the GSTP1 promoter in LNCaP cells. Cells were treated with 1.5 μM selenite and 5 μM 5-Aza-dC for 7 days or 0.2 μM TSA for 3 days. Total cellular protein or genomic DNA was isolated for western blot analysis or for ChIP assay. (A) Western blot analysis of protein levels of methylation and acetylation of Lys 9 on histone H3. (B) Western blot analysis protein levels of DNMT1. (C) ChIP assay of interaction of histone H3 or DNMT1 with the GSTP1 promoter. ChIP assay was performed with the anti-acetylated H3-Lys 9, anti-methylated H3-Lys 9 or anti-DNMT1 antibody. +Ab, with antibodies added; −Ab, no antibodies added; total input, total amount of sample used for ChIP assay. (D) Densitometric quantification of (C). Data are representative of three independent experiments.