Fig. 4.

Effects of selenite and 5-Aza-dC on genomic DNA methylation in LNCaP cells. Cells were treated with 1.5 μM selenite or 5 μM 5-Aza-dC for 7 days and total genomic DNA was isolated and blotted onto a nitrocellulose membrane. DNA methylation at 5-cytosine was detected using an anti-5-methylcytosine (5MeC) antibody and sample loading was determined by staining total DNA with methylene blue. The intensity of individual dots was measured by densitometry and levels of 5-methylcytosine were normalized by total DNA. Data are mean ± SD (n = 3). *P < 0.05 compared with control.