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Northern blot validation of microarray results. Total RNAs pooled from two rats at 1 or 20 weeks of the same treatments were separated on a denaturing gel and transferred to a membrane. The membrane was then probed for miRNAs or the control U6 snRNA. Results were analyzed using a PhosphorImager. Levels of the indicated miRNAs, after normalized to the U6 signals and compared with those of the control, 1-week RNA arbitrarily set at 100, were shown below the phosphorimages.
