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. Author manuscript; available in PMC: 2009 Nov 15.
Published in final edited form as: Cell. 2009 May 15;137(4):736–748. doi: 10.1016/j.cell.2009.04.023

Figure 1. Modulation of vascular flow affects HSC formation in zebrafish.

Figure 1

(A-M) Effect of blood flow modifiers on runx1/cmyb+ HSC formation. Zebrafish were exposed to chemicals (10 μM) from 10 somites to 36hpf and subjected to runx1/cmyb in situ hybridization. Photomicrographs were taken with Nomarski optics at 40× magnification. Representative examples following drug treatment are shown.

(L) Effect of todralazine (10 μM; 67 inc/84).

(M) Effect of drug treatment on runx1 expression, quantified by qPCR.

(N) Effect of drug treatment on the diameter of the dorsal aorta in vivo. Transgenic fli:GFP fish were treated with chemicals and imaged by confocal microscopy at 36hpf; all treatments were statistically significant from control, ANOVA, p<0.001, n=5).

HHS Vulnerability Disclosure