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. Author manuscript; available in PMC: 2009 Nov 15.
Published in final edited form as: Cell. 2009 May 15;137(4):736–748. doi: 10.1016/j.cell.2009.04.023

Figure 4. NO signaling affects zebrafish HSC formation independent of heartbeat.

Figure 4

(A-I) WT and sih-/- mutants were exposed to DMSO and SNAP (10 μM) from 10 somites to 36hpf.

(A-D) In situ hybridization for runx1/cmyb. SNAP rescues HSC formation in sih-/- mutants. (B) runx1/cmyb+ cells are highlighted by arrowheads.

(E) qPCR for runx1.

(F-I) Effect of heartbeat and SNAP on ephrinB2 expression, highlighted by arrowheads.

(J-U) Effect of L-NAME on HSC formation in embryos concurrently treated with blood flow modifying agents. L-NAME inhibits the effects of doxazosin (M, 7 inc/36 observed), metoprolol (O, 3 inc/31) and nifedipine (Q, 4 inc/28), but not of digoxin (S, 16 inc/29) and todralazine (U, 20 inc/33).

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