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. Author manuscript; available in PMC: 2009 Nov 15.
Published in final edited form as: Cell. 2009 May 15;137(4):736–748. doi: 10.1016/j.cell.2009.04.023

Figure 6. The effect of NO signaling on HSC development is cell-autonomous.

Figure 6

(A) Cells from cmyb:GFP transgenic donor embryos, injected with nos1 ATG MO or control MO, were transplanted into lmo2:dsRed recipients at the blastula stage.

(B) Donor contribution to HSC formation assessed by confocal microscopy at 36hpf. Shown are the merged picture on the top, red/green merge in the middle, and a high-magnification view of green fluorescence only on the bottom. cmyb:GFP donor-derived HSCs in recipients are highlighted by arrowheads.

(C) nos1 MO donors never contributed to HSC formation; presence of cmyb:GFP-derived donor cells in the eye is indicative of a successful transplant.

(D) HSC chimerism in transplanted embryos (control vs. nos1 MO, Fisher's exact, p=0.0065, n≥8).

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