Figure 7.

Chromatin remodeling at BDNF promoters during PFC development. (A) Illustration showing human BDNF gene, including its multiple exons and the three promoters (P1, P4 and P9, arrows) selected for chromatin immunoprecipitation studies (see text). (B and C) Developmental changes in tri-methylated histone H3-lysine 4 at BDNF P1 (B) and P4 (C). Bar graphs represent mean ± SEM of chip-to-input ratios (n = 3–6/age group). Notice significant increases in P1- and P4-associated histone methylation after birth or childhood [last two age groups merged in (C) due to limited number of samples], respectively. *P < 0.05, ANOVA post hoc Tukey.