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. 2008 Jul 16;17(20):3095–3104. doi: 10.1093/hmg/ddn206

Figure 9.

Figure 9.

A second D9 transgenic mouse line (F6) controls for effects of transient striatal DARPP-32 over-expression in DE5. (A) Quantitative RT–PCR on striatal tissue demonstrates equivalent levels of transgene mRNA (IRES) between DE5 mice (n = 3) and F6 mice (F6 het, n = 3). Predictably, levels in homozygous F6 mice (F6 hom, n = 3) are approximately twice as high. (B and C) Representative western blots demonstrating higher DARPP-32 striatal expression in 2 mo DE5 mice (P = 0.03) (B) and 2 mo F6 homozygotes (P = 0.003) (C) relative to age-matched NTG (non-transgenic) littermates. Over-expression of DARPP-32 was not apparent after 8.5 months of age in striatum (data not shown). (D) 14-month-old F6 mice possess normal gross morphology with open lateral ventricles (scale bar = 300 µm). (E) mRNA levels are unchanged in F6 in 14.5-month-old F6 heterozygote mice relative to non-transgenic mice. All values are expressed as mean ± SEM. *P < 0.05.