Figure 6.

p200 expression reduces the apparent affinity of β-catenin for TCF–GST and CAD–GST. (A) Western blot of a pull-down assay depicting the relative binding affinity of β-catenin for TCF–GST and CAD–GST fusion proteins. Lysates used in the pull-down assay were obtained from cells expressing β-catenin alone or β-catenin and p200/ p200Δ. Lysate loading represents 20 µg of protein. Input for GST-binding assays was 100 µg. (B) Semi-quantitative densitometric analysis of β-catenin band density from four independent experiments. Values represent arbitrary units (a.u.) calculated as the ratio of β-catenin binding to TCF–GST or CAD–GST under each of the transfection schemes relative to its level in the corresponding input and are presented as means ± SE. *P < 0.05 versus other respective treatment conditions.