Figure 4.

Loss of PC1 function induces mitotic catastrophe. (A) Immunohistochemical detection of cytochrome C (green) and caspase-3 (red) on DAPI (blue) counterstained DNA provided evidence of apoptotic activation during multipolar spindle separation only in PC1 knockdown cells but not in control cells. Original magnification ×1000. (B) 3 days post-transduction, mitoses (black bars) in apoptotic cells with diffuse cytochrome C staining and co-expression of caspase-3 (white bars) were estimated in five randomly chosen fields and reported as mean ± SD (n > 500 for each culture). *P = 0.003; **P = 0.0002 by two-tailed t-test with Welch correction (IMCD/P/siLuc versus IMCD/P/siPKD1₃₂₉₇).