Table 2.

Primer sequences for mutagenesis of rat NOTCH1

Missense mutation	Flanking primer	Mutagenic primer
G661S (5′ fragment)	5′ atgactgcccaggaaacaac	5′ caagatcgatAgctacgagtgtg
G661S (3′ fragment)	5′agggcagttgcatttgtacc	5′ cacactcgtagcTatcgatcttg
A683T (5′ fragment)	5′ atgactgcccaggaaacaac	5′ gacgaatgtAcgggcagcccctg
A683T (3′ fragment)	5′agggcagttgcatttgtacc	5′ caggggctgcccgTacattcgtc
R1279H (5′ fragment)	5′ tgagtccaacccttgtgtca	5′ ctgtgacccacAtggcacccag
R1279H (3′ fragment)	5′ tcattgaagttgagggagca	5′ ctgggtgccaTgtgggtcacag
A1343V (5′ fragment)	5′ tgagtccaacccttgtgtca	5′ cttcgagggtgTcacttgtg
A1343V (3′ fragment)	5′ tcattgaagttgagggagca	5′ cacaagtgAcaccctcgaag

Mutated nucleotides indicated in upper case.