Figure 6.
Eya1 gene dosage critically affects the expression of Sox2 in the otocysts. (A–D) Whole-mount in situ hybridization at E9.5 showing reduction of Sox2 expression in the otic vesicle (ov). Lower panels are sections through the otic region of whole-mount in situ embryos, which confirm the reduction of Sox2 expression specifically in the otic vesicle. (E–H) Section in situ hybridization showing reduction of Sox2 expression in the otic vesicle in the mutants. (I, J and L) Sections stained with Sox2 probe at E8.5 (I), E9.0 (J) and E9.5 (L). (K) Section stained with Eya1 probe at E9.0. (M) Section stained with X-gal for the Eya1lacZ allele at E9.5. (N) Section stained with anti-β-gal and Sox2 antibodies at E9.0. Scale bars: 100 µm.