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. Author manuscript; available in PMC: 2010 May 7.
Published in final edited form as: Org Lett. 2009 May 7;11(9):1883–1886. doi: 10.1021/ol701755k

Figure 1.

Figure 1

Gapped DNA synthesis assay with DNA pol β for dNTPs (dCTP, dATP, dTTP) and α,β-CXY analogues. The purity of the analogues was assessed from their failure to be inserted into a gapped DNA substrate. Primer (n) extension was assayed in the presence of low (L, 0.5 nM) or high (H, 50 nM) pol β and 1 mM analogue for 5 or 10 min, respectively. A: dCTP vs. 6; B: 3 vs. 11, 9, dATP and dTTP. The mobility of the extension product (n+1) with dCTP, dATP or dTTP serves as a reference, where most of the primer (200 nM) is extended after a 21 min incubation with 50 nM enzyme and 100 µM dNTP.