. Author manuscript; available in PMC: 2009 Aug 7.

Published in final edited form as: Biotechniques. 2009 Mar;46(3):175–182. doi: 10.2144/000113074

Table 1.

Optimized Parameter Values for Phage pVIII Modification Reactions

Reagent	Q/k₁	k_h/k₁ (µM)	k₂/k₁
NHS-PEO₄-biotin	1.080	1274.1	0
sulfo-NHS-AF680	0.2243	2054.5	0

The kinetic parameters in the last three columns were adjusted so as to minimize the overall deviation of the observed modification levels for the test reactions at various protein and initial reagent concentrations from the corresponding theoretical modification levels calculated with Equation 1 for those same protein and initial reagent concentrations. The metric for overall deviation was the sum of the squares of the logarithms of the ratios of observed to theoretical modification levels.