Table 5.
Cycle visit blood and urine specimen collection
| Collection tube (anticoagulant) (no. tubes × volume) |
Specimen type | No. of aliquots × volumea |
Test to be performedb | Methodb | Testlabc |
|---|---|---|---|---|---|
| Lavendar top (EDTA) (2 × 10 mL) |
Whole blood | 1 × 0.15 | Total haemoglobin (Hgb) | Cyan met haemoglobin method |
UB |
| Whole blood | 0.15 mL stabilised into 1.35 mL PCA |
Thiols (e.g. glutathione, glutathione disulphide) |
Stored future use | UB | |
| EDTA plasma | 1 × 1.25 mL | F2-8-isoprostanes | GC-MS | U Minn | |
| EDTA plasma | 1 × 1.1 mL | Lipid peroxidation profile | HPLC | UB | |
| EDTA plasma | 1 × 0.75 mL | TBARS | Spectrofluorometry | UB | |
| EDTA plasma | 1 × 0.75 mL | NMR lipoproteins (1 per cycle) |
NMR | Liposcience | |
| EDTA plasma | 1 × 1.1 mL | Homocysteine (3 per cycle) |
CL-EIA | Kalieda | |
| EDTA plasma | 1 × 1.25 mL | Extra stored | |||
| EDTA plasma | 8 × 0.6 mL | Extra stored | |||
| Buffy coat | Stored buffy coat | Future DNA extraction | |||
| Green top (sodium heparin) (1×5 mL) |
Heparin plasma | 0.5 stabilised into 2.0 mL MPA |
Vitamin C | DNPH Colorimetric assay | UB |
| Heparin plasma | 1 × 0.3 mL | Plasma glutathione peroxidase |
Kinetic enzyme assay | UB | |
| Heparin plasma | 3 × 0.6 mL | Extra stored | |||
| Buffy coat | 1 × 0.5 mL | Stored for DNA extraction | |||
| Washed erythrocytes |
1 × 0.5 mL | Antioxidant enzymes (SOD, GPx, GSHR) |
Kinetic enzyme assay | UB | |
| Red top (no anticoagulant) (1 × 10 mL; 1 × 15 mL) |
Serum | 1 × 0.3 mL | PON | Kinetic enzyme assay | UB |
| Serum | 1 × 1.1 mL | Fat soluable vitamins | HPLC | UB | |
| Serum | 1 × 1.25 mL | CMP | Auto chemistry analyser | Kalieda | |
| Lipid profile | Auto chemistry analyser | Kalieda | |||
| Uric acid | Colorimetry | Kalieda | |||
| Bilirubin | Colorimetry | Kalieda | |||
| hsCRP | CL-EIA | Kalieda | |||
| Serum | 1 × 0.6 mL | Oestradiol | Radio immunoassay | Kalieda | |
| Serum | 1 × 2.0 mL | Insulin, progesterone, LH, FSH, SHBG |
CL-EIA | Kalieda | |
| Serum | 8 × 0.6 mL | Extra stored | |||
| Urine | Spot urine | 7 × 4.0 mL | Extra stored | ||
| Cytokines | Luminex flow cytometry | Gainesville |
PCA, perchloric acid stabilising solution; MPA, meta-phosphoric acid stabilising solution.
CL-EIA, DPC Inc., Immunlite 2000 Solid phase competitive Chemiluminescent Enzymatic Immunoassay; DNPH, dinitrophenyl hydrazine; EDTA, ethylenediamine tetraacetic acid; FSH, follicle-stimulating hormone; GC-MS, gas chromatograph-mass spectrometry; GPx, glutathione peroxidase; GSHR, glutathione reductase; hsCRP, high sensitivity C-reactive protein; LH, lutinising hormone; NMR, nuclear magnetic resonance spectroscopy; PON, paraoxinase; SHBG, sex hormone binding globulin; SOD, superoxide dismutase; TBARS, thiobarbituric acid reactive substances.
UB, Dr Richard Browne, University at Buffalo, Buffalo NY; Kaleida Health Laboratories, Buffalo NY; U Minn, Dr Myron Gross, University of Minnesota, Minneapolis, MN; Liposicence, Raleigh, NC; Gainesville, Dr Chegini, U Florida, Gainesville, FL. Note blood draw order was: 15 mL red, 10 mL lavender, 4 mL green, 10 mL lavender, 10 mL red.