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. Author manuscript; available in PMC: 2009 Oct 30.
Published in final edited form as: Oncogene. 2009 Mar 30;28(17):1892–1903. doi: 10.1038/onc.2009.34

Figure 2.

Figure 2

Expression of selected genes in the 8p12 amplicon. Transcript levels for each gene were determined by quantitative RT-PCR and normalized to GUSB. The PCR reaction conditions and primers and probes for each gene are given in Supplementary Table 4. The data are displayed as expression levels relative to MCF10A (histograms) or as expression relative to GUSB if no expression was detected in MCF10A (FKSG2, PLEKHA2, TM2D2, ADAM18, ADAM2, ZMAT4). No expression of HTRA4 was detected. Protein expression levels were determined if suitable antibodies were available (Supplementary Table 5). Western blots are shown below the RT-PCR histograms. Equal loading of lanes was confirmed by expression of β-actin (data not shown). Genes are displayed according to inclusion in 8p amplicons A1-A4 and the copy number of the locus in each cell line is shown below the name of the cell line (white = average copy number, light gray = gain, and dark gray = amplification).