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. 2009 Jun 4;18(17):3266–3273. doi: 10.1093/hmg/ddp264

Figure 2.

Figure 2.

Trans-splicing modulation of E10 usage in tau transcripts. COS cells were co-transfected with the LI9/LI10 minigene and TauPTM6 or TauPTM9. (A) RT–PCR analysis with the forward primer VctF, specific for the target minigene and the PTM-specific reverse primer FLAGR, complementary to the FLAG epitope sequence, demonstrates trans-splicing between minigene transcripts and PTMs. The difference in size between the products generated using Tau PTM6 and TauPTM9 corresponds to E10. (B) Change in E10 inclusion after co-transfection of LI9/LI10 with TauPTM6 and TauPTM9. Both cis- and trans-spliced products were detected by RT–PCR with the forward primer VctF and the reverse primer 11R, corresponding to an exon 11 sequence. Co-transfection with TauPTM6 or TauPTM9 resulted in an increase or decrease of E10 inclusion, respectively.