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. 2009 Jun 4;18(17):3266–3273. doi: 10.1093/hmg/ddp264

Figure 3.

Figure 3.

Splicing pattern of minigenes containing FTDP-17 mutations. (A) Structure of the MAPT minigenes incorporating the exonic N279K and Δ280K mutations or the intronic DDPAC +14 mutation. The backbone minigene, TauEx9-11, consists of tau exons 9–10–11 and 498 bp 5′ and 264 bp 3′ of intronic sequences flanking E10. (B) Splicing was assayed by RT–PCR using primers VctF and 11R in transfected COS cells. TauEx9-11WT produces mainly E10+ RNA; the mutants minigenes, TauEx9-11DDPAC and TauEx9-11N279K, produce exclusively E10+ RNA, whereas TauEx9-11Δ280K produces an excess of E10− RNA. Arrows indicate the locations of the PCR primers used to detect splice isoforms.