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. 2009 Jun 4;18(17):3266–3273. doi: 10.1093/hmg/ddp264

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Absence of recombination between minigene and PTM at the DNA level. RNA isolated from cells co-transfected with TauEx9-11^DDPAC and TauPTM6 or TauPTM9 was analyzed by PCR with (+RT) or without (−RT) prior reverse transcription. No cis- or trans-splicing (A and B) products were detected without RT. (A) Trans-splicing products only are amplified using the VctF-FLAGR primer combination; no larger size species were detected with or without RT. (B) In contrast, in addition to cis- and trans-splicing products, minigene DNA was readily amplified from the traces of DNA present in the RNA preparation using the VctF–11R primer combination.