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. 2009 Jun 4;18(17):3274–3285. doi: 10.1093/hmg/ddp265

Figure 7.

Figure 7.

SNCA-Rep1 expansion does not lead to detectable SNCA protein concentration differences in mouse blood. (A) Quantification of human SNCA protein in transgenic mouse blood homogenates using sandwich ELISA [hSA-3/211-B] and 384-well plate format (triplicates; 50 µl/well); a total of 72 mice were analyzed. Signals were specific for human SNCA protein because whole blood from WT and snca-knockout mice did not generate ELISA signals above the background (Supplementary Material, Fig. S4A). Values were then corrected for the calculated gene copy number in each mouse line and grouped according to the three genotypes examined, i.e. Rep1-261 bp; Rep1-259 bp; Δ-Rep1 (ratio: female:male, 50:50 in all lines). (B) Quantification of total SNCA protein concentration levels in transgenic mouse blood was carried out with sandwich ELISA [hSA3/Syn1-B] and 384-well plates. This ELISA measures both mouse snca and human SNCA proteins (Supplementary Material, Fig. S4B); values (in ng/µl) were then corrected for gene copy number.