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. 2009 Jun 5;18(17):3286–3297. doi: 10.1093/hmg/ddp266

Figure 5.

Figure 5.

Granule cell migration defects are rescued with DISC1 overexpression. In utero electroporation following co-injection of human DISC1 and Disc1 shRNA3 (5:1 ratio) into the mouse hippocampus at E15–E19 rescues the deficits in granule cell migration observed with shRNA3 injection alone. (A) Representative image showing that cells receiving hDISC1 + shRNA3 are able to migrate to the dentate gyrus. (B) Quantification of cells reaching each migration zone shows that a significantly greater percentage of cells reaches zone 3 following the injection of hDISC1/shRNA3 (27.6%) than shRNA3 alone (8.1%). Similarly, a significantly lower percentage of cells remains in zone 1 following hDISC1/shRNA3 injection (45.1%) than with shRNA3 alone (70.4%). Interestingly, a significantly greater percentage of cells migrates to zone 3 after hDISC1/shRNA3 injection (27.6%) compared with mshRNA3 (18.8%). (C) When the ratio of hDISC1:shRNA3 injected was lowered from 5:1 to 2:1, the percentage of cells migrating to zone 3 was no longer significantly different from mshRNA3 injection (20.3% versus 18.8%). Values are presented as mean ± SEM. n = 4 (hDISC1 + shRNA3 5:1), n = 5 (hDISC1 + shRNA3 2:1), n = 9 (mshRNA3), n = 13 (shRNA3). *P < 0.05; ***P < 0.0001.