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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Cell Metab. 2009 Aug;10(2):89–98. doi: 10.1016/j.cmet.2009.06.011

Figure 4. Neurotransmitter content of LHA LepRb Neurons.

Figure 4

(A,B) Immunofluorescent detection of EGFP (green) and MCH (A, red) or OX (B, red) in the LHA of colchicine-treated LepRbEGFP mice. (C,D) Immunohistochemical detection of pSTAT3-IR (green nuclei, pseudocolored) and MCH (C, red) or OX (D, red) following ICV leptin treatment for 1 hr. (E) Immunohistochemical detection of pSTAT3 (red, pseudocolored) and immunofluorescent detection of EGFP (green) in Gad1EGFP mice. (F) Digital zoom of (E). (G,H) immunofluorescent detection of EGFP (green) and OX (red (G)) or MCH (red (H))in mice that express EGFP in GAD67 neurons. Scale bars = 10 µm. 3V= third cerebral ventricle; f= fornix