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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Cell Metab. 2009 Aug;10(2):89–98. doi: 10.1016/j.cmet.2009.06.011

Figure 5. Ad-iZ/EGFPf-mediated tracing reveals projection of LHA LepRb neurons to the VTA.

Figure 5

(A) Schematic diagram showing cre-mediated expression of EGFPf in infected LepRb-expressing neurons following stereotaxic injection of Ad-iZ/EGFPf into Leprcremice. (B) Summary of Ad-iZ/EGFPf injection sites for the eleven (11) cases utilized for the study. (C) Immunofluorescent detection of EGFPf (green) and MCH (red) in the LHA of an example of a correctly targeted intra-LHA injection of Ad-iZ/EGFPf in the Leprcremice. (D) Immunofluorescent detection of EGFPf-containing projections in the VTA of Leprcre mice following intra-LHA injection of Ad-iZ/EGFPf. (E) Higher magnification of (D). Scale bars = 10 µm. VTA= ventral tegmental area; SNc- substantia nigra pars compacta; IP= interpeduncular tubercle.