Table 2.
Genetic interaction tests
|
ASEL
|
ASER
|
|||
|---|---|---|---|---|
| Genotype | % animals with lim-6::gfp* | % animals with gcy-5::gfp† | % animals with lim-6::gfp | % animals with gcy-5::gfp |
| Wild type | 100 (n>100) | 0 (n>100) | 0 (n>100) | 100 (n>100) |
| lsy-9(ot85)‡ | 100 (n=68) | 0 (n=97) | 44 (n=68) | 67 (n=97) |
| lsy-9(ok631)§ | 100 (n=50) | 0 (n=82) | 64 (n=50) | 36 (n=82) |
| lsy-6(ot71) | 0 (n=25) | 100 (n=43) | 0 (n=25) | 100 (n=43) |
| lsy-6(ot71); lsy-9(ot85)‡ | 22 (n=52) | nd | 21 (n=52) | nd |
| lsy-6(ot71); lsy-9(ok631)§ | 67 (n=60) | 43 (n=61) | 67 (n=60) | 55 (n=61) |
| die-1(ot26) | 0 (n=50) | 100 (n=50) | 0 (n=50) | 100 (n=50) |
| die-1(ot26); lsy-9(ot85)‡ | 0 (n=34) | nd | 0 (n=34) | nd |
| die-1(ot26); lsy-9(ok631)§ | nd | 100 (n=30) | nd | 100 (n=30) |
| cog-1(ot123) | nd | 100 (n=50) | nd | 100 (n=50) |
| cog-1(ot123); lsy-9(ok631)§ | nd | 56 (n=105) | nd | 74 (n=105) |
nd, not determined.
*
Transgene used was otls114 (lim-6prom::gfp).
†
Transgene used was ntls1 (gcy-5prom::gfp).
‡
ceh-36::rfp (otls151) is in the background and only those animals that showed normal ceh-36::rfp expression were scored. Animals showed consistent numbers for L1 and adult scoring. L1 numbers are presented.
§
che-1::mCherry (otls217) is in the background and only those animals that showed normal che-1::mCherry expression were scored. These animals were scored as L1s due to lethality of ok631