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. 2009 Aug 7;136(17):2965–2975. doi: 10.1242/dev.036616

Fig. 3.

Fig. 3.

Molecular identity of ectopic progenitors in Marcks-/- cortex. (A-F) Immunolabeling of E15.5 radial progenitors in WT (A,D) and Marcks-/- (B,E) cortex with anti-PAX6 and anti-SOX2 antibodies illustrates the presence of ectopic PAX6+ (bracket, B) and SOX2+ (arrows, E) progenitors within the IZ/CP in Marcks-/- cortex. These ectopic PAX6+, TBR2+ and SOX2+ progenitors within the IZ/CP co-label with radial-glia-specific anti-GLAST antibodies (arrows, C,F; arrowhead in panel C indicates a GLAST+ cell labeled with both PAX6 and TBR2). (G,H) Radial and intermediate progenitors in WT and Marcks-/- cortex were immunolabeled with anti-PAX6 and anti-TBR2 antibodies. Compared with WT (G), PAX6+ and TBR2+ cells are displaced away from the VZ/SVZ region in Marcks-/- cortex (arrows, H). (I,J) Quantification of PAX6+ and TBR2+ cell density (104/mm2) in the upper cerebral wall indicates the extent of ectopic placement of progenitors in Marcks-/- cortex (I). The width of PAX6+ and TBR2+ cell layers in the VZ is significantly reduced in Marcks-/- cortex (compare brackets in G and H; quantified in J). Data shown are mean±s.e.m.; asterisk indicates significance when compared with controls at P≤0.01. Scale bar in H: 100 μm for A,B,D,E; 20 μm for C,F; 50 μm for G,H; 75 μm for I. CP, cortical plate; IZ, intermediate zone; VZ, ventricular zone.