Table 1.

Oncolytic activity of ReoT3D in 9 NSCLC cell lines included in the NCI-60 cell line panel

NSCLC Cell Line	ED50 (log10 pfu/cell: mean ± SD)
NCI-H460*	2.68 ± 0.25
A549/ATCC*	2.51 ± 0.27
HOP-62*	2.56 ± 0.19
NCI-H322M	> 3.30
NCI-H226	> 3.30
EKVX	2.47 ± 0.34
NCI-H23*	2.47 ± 0.15
NCI-H522	1.46 ± 0.12
HOP-92	1.96 ± 0.27

ED50: 50% effective dose defined by the initial MOI (pfu/cell) to achieve 50% cell death in 48 hours after infection. Shown are mean ± SD from 3 independent experiments. *Asterisk denotes cell lines with ras gene mutation. Abbreviations: ReoT3D, reovirus type 3 Dearing; NSCLC, non-small cell lung cancer; MOI, multiplicity of infection; pfu, plaque forming units.