Figure 4.

Effect of presynaptic dialysis of EGTA in PV+/+ mice. (A) Superimposed IPSCs recorded in a Purkinje cell (dotted traces, post) of a PV+/+ mouse after induction of two action potentials in a presynaptic interneuron by depolarizing steps (30 ms ISI) and average response for the entire experiment (solid trace). The interneuron was recorded with a solution containing 50 μM EGTA. (B) Representative results from a similar experiment where the interneuron was recorded with a solution containing 1 mM EGTA. (C) Average PPR as a function of ISI in PV+/+ mice in conditions where interneurons were extracellularly stimulated (open circles, same data as in Fig. 2C), or were recorded with a solution containing 50 μM EGTA (filled circles, n = 11), or were recorded with a solution containing 1 mM EGTA (filled diamonds, n = 9).