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. 2009 Jul 14;4:28. doi: 10.1186/1750-1326-4-28

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Copyright © 2009 Tamayev et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Quantitative analysis of protein-protein interactions using Biacore assay (surface plasmon resonance biosensor assay). Strep-tagged AID peptides and Strep-tag control peptide were immobilized on sensor chips CM5 (Biacore). Binding curves for GST-Grb2, Numb p71, and X11β proteins were expressed in resonance units (RU) as a function of time. (A) AID^pY and GST-Grb2 interaction, (B) AID^pT^pY and GST-Grb2 interaction, (C) AID and GSTNUMB-p71 interaction, (D) AID^pY and GST-NUMB-p71 interaction, (E) AID^pT^pY and GST-NUMB-p71 interaction, (F) AID and X11β interaction, (G) AID^pT and X11β interaction, and (H) AID^pT^pY and X11β interaction. The kinetic parameters and concentrations of the analytes are indicated in A-H.