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Surface plasmon resonance measurements. An amtB promoter fragment spanning nucleotides -186 to -156 (relative to the start codon) was immobilized on Biacore chips rising concentrations of protein were added (for colour code, see Fig. 3A). (A) Binding properties of negative control (MBP) and positive control (AmtR-MBP fusion), (B) Influence of amino acid exchanges on binding of AmtR variants. Colour code for the concentrations of proteins added as in (A).
