Table 1.
Selected proteins with altered abundance under H2 limitation.
| ORF # | Function | Average log2 ratioa |
|---|---|---|
| Methanogenesis | ||
| MMP0820 | FrcA, coenzyme F420-reducing hydrogenase | 1.30 ± 0.56 |
| MMP1382 | FruA, coenzyme F420-reducing hydrogenase | 0.77 ± 0.16 |
| MMP1384 | FruG, coenzyme F420-reducing hydrogenase | 0.88 |
| MMP1385 | FruB, coenzyme F420-reducing hydrogenase | 0.90 |
| MMP1460 | EhaM, energy-conserving hydrogenase A | 0.56 ± 0.08 |
| MMP1463 | EhaP, energy-conserving hydrogenase A polyferredoxin subunit | 0.64 ± 0.27 |
| MMP0058 | Mer, methylenetetrahydromethanopterin reductase | 0.58 |
| MMP1245 | FwdF, formylmethanofuran dehydrogenase | 0.20 |
| MMP1247 | FwdD, formylmethanofuran dehydrogenase | 0.23 |
| MMP1248 | FwdA, formylmethanofuran dehydrogenase | 0.27 |
| MMP1249 | FwdC, formylmethanofuran dehydrogenase | 0.28 ± 0.07 |
| MMP1697 | HdrA, heterodisulfide reductase | 0.35 ± 0.18 |
| MMP1696 | VhuD, F420 non-reducing hydrogenase | 0.35 ± 0.11 |
| MMP1695 | VhuG, F420 non-reducing hydrogenase | 0.34 |
| MMP1694 | VhuA, F420 non-reducing hydrogenase | 0.29 |
| MMP0372 | Mtd, F420-dependent methylenetetrahydromethanopterin dehydrogenase | 0.35 ± 0.10 (0.89)b |
| MMP1054 | HdrC2, heterodisulfide reductase | 0.33 |
| MMP1053 | HdrB2, heterodisulfide reductase | 0.33 ± 0.11 |
| MMP1563 | MtrB, methyltransferase | 0.27 ± 0.16 |
| MMP1564 | MtrA, methyltransferase | 0.09 |
| MMP0127 | Hmd, H2-dependent methylenetetrahydromethanopterin dehydrogenase | -2.08 (-3.57)b |
| MMP0125 | Hypothetical protein | -1.19 |
| MMP0875 | S-layer protein | -1.25 |
| MMP1176 | Putative iron transporter subunit | -0.83 |
| MMP1206 | GlnA, glutamine synthetase | -0.35 |
aAverage of four log2 ratios: 15N-labeled H2-limited compared with 14N-labeled nitrogen-limited, 14N-labeled H2-limited compared with 15N-labeled nitrogen-limited, 15N-labeled H2-limited compared with 14N-labeled phosphate-limited, and 14N-labeled H2-limited compared with 15N-labeled phosphate limited. Standard deviations are given. Where protein abundance was affected by a second nutrient limitation (other than H2), the average is from two ratios only, each H2-limited compared with the non-affecting nutrient limitation.
bValues in parentheses represent measurements of mRNA by qRT-PCR.