Table 2.
Selected proteins with altered abundance under nitrogen limitation.
| ORF # | Function | Average log2 ratioa |
|---|---|---|
| Nitrogen fixation | ||
| MMP0853 | NifH, nitrogenase reductase | 2.29 ± 0.16 |
| MMP0854 | NifI1 | 1.68 ± 0.57 |
| MMP0855 | NifI2 | 2.10 ± 0.23 |
| MMP0856 | NifD, nitrogenase | 2.45 ± 0.15 |
| MMP0857 | NifK, nitrogenase | 2.03 ± 0.22 (7.09)b |
| MMP0858 | NifE | 1.85 ± 0.42 |
| MMP0859 | NifN | 1.65 ± 0.30 |
| MMP0860 | NifX | 3.13 ± 0.60 |
| MMP0446 | NifX-NifB superfamily | 1.05 ± 0.40 |
| Ammonia transport and regulation | ||
| MMP0064 | GlnK1 | 1.30 |
| MMP0065 | AmtB1 | 2.81 ± 0.31 |
| MMP0066 | GlnB | 0.45 ± 0.41 |
| MMP0067 | GlnK2 | 1.59 ± 0.48 |
| Ammonia assimilation | ||
| MMP1206 | GlnA, glutamine synthetase | 1.23 |
| Molybdate transport | ||
| MMP0205 | ModA, molybdate binding protein | 2.11 ± 0.47 |
| MMP0507 | ModA, molybdate binding protein | 2.24 ± 0.50 |
| MMP0516 | ModD, molybdate transporter subunit | 0.73 ± 0.23 |
aAverage of four log2 ratios: 14N-labeled nitrogen-limited compared with 15N-labeled H2-limited, 15N-labeled nitrogen-limited compared with 14N-labeled H2-limited, 15N-labeled nitrogen-limited compared with 14N-labeled phosphate-limited, and 14N-labeled nitrogen-limited compared with 15N-labeled phosphate limited. Standard deviations are given. Where protein abundance was affected by a second nutrient limitation, average is from two ratios only, each nitrogen-limited compared with the non-affecting nutrient limitation.
bValue in parentheses represents measurements of mRNA by qRT-PCR.