Table 1.

Quantification of FM4-64 uptake

	Mean percentage of cells displaying punctate or vacuolar staining ± s.e.m.
	10-minute chase			45-minute chase
	Punctate	Vacuolar	Other	Punctate	Vacuolar	Other
WT (empty vector)	3±1	89±7	9±4	2±1	88±2	10±2
tlg2Δ (empty vector)	65±8	22±3	11±5	8±5	87±7	6±2
tlg2Δ (HA-Tlg2p)	16±3	68±5	14±3	7±3	83±5	11±4
tlg2Δ (HA-Sx16)	15±4	74±8	8±4	4±1	88±5	9±5
tlg2Δ (HA-Sx4)	59±9	29±4	13±3	3±3	86±5	12±2

Note that vacuolar staining represents either the single large vacuole observed in wild-type cells or the fragmented vacuoles observed in tlg2Δ cells. WT, wild type