Table 3.
Plasmids used in this study
| Plasmid | Description | Reference |
|---|---|---|
| pCOG070 | Yeast expression plasmid (2μ, URA3) encoding wild-type HA-Vps45p | (Carpp et al., 2006) |
| pCOG071 | As pCOG070, encoding HA-Vps45pL117R | (Carpp et al., 2006) |
| pCOG022 | E. coli expression vector encoding two IgG-binding domains of protein A (PrA) | (Carpp et al., 2006) |
| pCOG025 | E. coli expression vector encoding Tlg2p-PrA | (Carpp et al., 2006) |
| pALA1 | E. coli expression vector encoding Sx16-PrA | This study |
| pFB09/1 | E. coli expression vector encoding Sx4-PrA | This study |
| pNB701 | Yeast expression plasmid (CEN, URA3) encoding Pho8p from the CUP1 promoter | This study |
| pMAZ002 | Yeast expression plasmid (CEN, URA3) encoding HA-Sx16 from the CUP1 promoter | This study |
| pMAZ007 | Yeast expression plasmid (CEN, URA3) encoding HA-Sx4 from the CUP1 promoter | This study |
| pMAZ006 | Yeast expression plasmid (CEN, URA3) encoding HA-Tlg2p from the CUP1 promoter | This study |
| pSL2099 | Yeast expression plasmid (CEN, LEU2) encoding Ste3p-Myc from the GAL1 promoter | (Davis et al., 1993) |
| pVT102U | Yeast expression plasmid (2 μ, URA3) harbouring the ADH1 promoter | (Vernet et al., 1987) |
| pSGS043 | Yeast expression plasmid (2 μ, URA3) encoding Tlg2p lacking the N-terminal 230 residues (Tlg2pΔHabc) from the ADH1 promoter | This study |
| pSGS044 | Yeast expression plasmid (2 μ, URA3) encoding Sx16 lacking the N-terminal 205 residues (Sx16ΔHabc) from the ADH1 promoter | This study |
| pCMD008 | E. coli expression vector encoding Tlg2p-GST | This study |
| pCMD007 | E. coli expression vector encoding Tlg2pΔHabc-GST | This study |
| pCMD004 | E. coli expression vector encoding a chimeric SNARE motif made from Tlg1p and Sx8 as described by Paumet et al. (Paumet et al., 2005) tagged with GST | This study |
| pJM132 | E. coli expression vector encoding His6-Vti1p | (McNew et al., 2000) |
| pJM81 | E. coli expression vector encoding His6-Snc2p | (McNew et al., 2000) |
| pNB710 | E. coli expression vector encoding His6-Vps45p | (Carpp et al., 2006) |
A detailed account of plasmids made during the course of this study can be found in the Materials and Methods