Fig. 7.

p110α inhibition does not prevent loss of self-renewal or block mESC differentiation. (A) mESCs were treated with the p110α inhibitors 15e or PIK-75 for 4 days, at the doses indicated, in the presence or absence of LIF. The average percentage of alkaline-phosphatase-positive colonies, with s.e.m. (n=3), is shown, ^***P<0.001 following a Student's t-test. (B) The average number of self-renewing alkaline-phosphatase-positive and non-self-renewing colonies in each condition is shown with s.e.m. (n=3). (C) mESCs cultured in the presence of LIF were treated with TGX-221 for 4 days in the presence or absence of 600 nM 15e. Self-renewal was assessed by staining for alkaline-phosphatase activity and the average percentage of alkaline-phosphatase-positive colonies, with s.e.m., are shown (n=4). ANOVA (P=0.002) and Dunnett's post-hoc test were applied; ^**P<0.01.