Skip to main content
. 2008 Oct 22;28(43):10885–10892. doi: 10.1523/JNEUROSCI.3015-08.2008

Figure 1.

Figure 1.

Expression of SWSR133A in the nervous system of sws1 mutant flies does only partially rescue the degenerative phenotype and affects esterase activity. A, A sws1 fly shows the characteristic vacuolization in the neuropil (arrows). B, Expressing SWSR133A pan-neuronally in these flies leads to a significant reduction in vacuole formation. C, Expressing wild-type SWS under the same conditions almost completely reverts the mutant phenotype. D, Mean area of vacuoles in μm2 in the three genotypes shown in A, B, and C. Number of measured flies was as follows: n = 24 for Appl-GAL4, sws1 (A), n = 28 for Appl-GAL4, sws1; UAS-SWSR133A (B), and n = 34 for Appl-GAL4, sws1; UAS-SWS (C). SEMs are indicated. All sections are horizontal paraffin sections through the heads of 14-d-old flies. E, Esterase activity in fly head homogenates revealed that expressing the SWSR133A construct pan-neuronally did not restore the esterase function in sws1. In contrast, this construct showed esterase function in a wild-type background and heterozygous sws1/+ flies, approximately doubling the activity. All values are expressed relative to wild type (100%). SEMs are indicated. Scale bar: (in A) A–C, 50 μm. la, Lamina; me, medulla; lo, lobula; lb, lobula plate; cb, central brain.