Oligonucleotide primers designed to specifically amplify Kv1.4, Kv1.5 and Kv4.5 channels were used in RT-PCR reactions from the rat ganglia and, for comparison, brain Poly A+ RNA. As a control, first-strand cDNA reactions were performed with (+) or (−) reverse transcriptase (RT). In both reactions, brain and ganglia, the (–)RT lanes had no signal. Expected size of the cDNA fragments was 543bp, 414bp and 690bp for Kv1.4, Kv1.5 and Kv4.3 respectively.