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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Nat Med. 2009 May 17;15(8):901–906. doi: 10.1038/nm.1967

Figure 5.

Figure 5

Anti-immunoadhesin antibodies in immunized monkeys. (a) Serum (1:100 dilution) collected over time from each immunized animal was tested by ELISA for reactivity against the homologous transgene. For example, sera from 5L7 recipients 05C002, 05C004, and 05C053 were reacted against purified 5L7 protein (as indicated on the x-axis). The dashed line indicates the limit of sensitivity. Animals with positive homologous reactivity were 5L7 recipients 05C004 and 05C053, and N4 recipient 05D043. The inset shows sera from animal 05C004 at a 1:1000 dilution, demonstrating a peak of activity at eight weeks, and a significant waning of the response by week 32. (b) Sera from the three animals with homologous reactivity were tested against all three purified proteins (as indicated on the x-axis). As expected, each animal serum reacted with the homologous transgene. 05C004 reacted against 5L7 (homologous) and 4L6 (heterologous). 05C053 reacted against 5L7 (homologous), 4L6, and N4 (both heterologous). 05D043 reacted only with the homologous N4 protein. The dashed line indicates the limit of sensitivity.