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. Author manuscript; available in PMC: 2009 Aug 7.
Published in final edited form as: Alcohol Clin Exp Res. 2009 May 4;33(8):1450–1459. doi: 10.1111/j.1530-0277.2009.00975.x

Figure 4. Superoxide dismutase isozymes and catalase in rat plantaris.

Figure 4

Real time PCR analyses of three superoxide dismutase isozymes (i.e., Cu/Zn-SOD1, Mn-SOD2, and Cu/Zn-SOD3) and catalase were performed in plantaris muscles from rats chronically fed alcohol (EtOH) for 35 weeks. Cu/Zn-SOD1 and Cu/Zn-SOD3 were induced in plantaris muscles from alcohol-fed rats while Mn-SOD2 mRNA was decreased (Panels A, C and B, respectively). Procysteine stimulated the production of Cu/Zn-SOD1 and Mn-SOD2 in alcohol-fed rat plantaris muscles (A and B, respectively). (D) Expression of the hydrogen peroxide scavenging enzyme, catalase, was unaffected by chronic alcohol ingestion, however, mRNA levels were induced following 12 weeks of procysteine supplementation. Data are represented as means ± range of potential values based on the 2-ΔΔCT method (Otis et al., 2007; Otis et al., 2008a, b) and expressed as fold changes relative to controls (n=6-7 rats/group). Significance was accepted at p ≤ 0.05. *, compared to control group. #, compared to EtOH group.