Figure 5. Interactions of DCs and CD4⁺ T cells in post-healing biopsies during acyclovir treatment.

(a) Dual immunofluorescence staining of CD123 with CD3 showing the interaction of CD123⁺ cells (green) and CD3⁺ T cells (red) in a week 12 post-healing biopsy while on acyclovir therapy. (b) Associations of CD4⁺ cells (green) with cells expressing DC-SIGN (red). Biopsy was taken after 20 weeks of acyclovir therapy. (c) Detailed micrographs illustrate direct interaction between CD123⁺ DCs and CD4⁺ T cells, and DC-SIGN⁺ DCs and CD4⁺ T cells at genital lesion site post healing. Panel I shows CD123⁺ DC are contiguous to CD4⁺ T cells. Panel II shows interactions between CD123⁺ DC cells and T cells. Panel III illustrates clusters of CD3⁺CD4^high T cells and CD3^-CD4^low DCs. Panel IV shows a CD4⁺ T cell contiguous to a DC-SIGN expressing cell scale bar:50μ(a), 100μ(b), 20μ(c).