Figure 2.

Gross abnormalities in lp_A1^(−/−) mice. (a) Craniofacial deformity in adult lp_A1^(−/−) mice. Note the blunted snout and more widely spaced eyes. (b) Overall size decrease in lp_A1^(−/−) mice. Two 9-week-old male siblings are shown. (c) Frontal cranial hemorrhage in a newborn lp_A1^(−/−) pup but not in littermates. (d) Sagittal sections of an E13 lp_A1^(−/−) embryo with frontal cranial hemorrhage (the earliest time point this phenotype was observed). Sections shown were stained to identify erythrocytes (left) using peroxidase (pseudocolored red) or Nissl substance (right) using cresyl violet. (e) Total body mass measurements for lp_A1^(−/−) mice (●) and control lp_A1^(+/−) littermates (○) from lp_A1^(+/−) X lp_A1^(+/−) crosses. Data points are means ± SD; *, P < 0.05 (repeated measures were analyzed with ANOVA). Mass measurements of lp_A1^(+/+) littermates overlapped those shown for lp_A1^(+/−) and are not presented for clarity.