Skip to main content
. 2000 Nov 21;97(24):13390–13395. doi: 10.1073/pnas.97.24.13390

Figure 1.

Figure 1

Effect of PACAP on apoptosis of cerebellar granule cells. (A and B) Typical microphotographs illustrating the effect of PACAP on survival and neurite outgrowth. Cells were cultured for 48 h in the absence (A) or presence (B) of 10−7 M PACAP. Living cells were labeled with FDA (green fluorescence), and dead cells were labeled with propidium iodide (red fluorescence). Scale bar = 10 μm. (C) Effect of graded concentrations of PACAP (10−11 to 10−6 M; 48 h) on survival of cultured cells. (D and E) Microphotographs illustrating the effect of PACAP on DNA fragmentation on cultured cells. Cells were cultured for 24 h in the absence (D) or presence (E) of 10−7 M PACAP, and DNA fragmentation was labeled by the TUNEL technique. Scale bar = 20 μm. (F) Representative gel electrophoresis illustrating the effect of PACAP on DNA laddering. MW, molecular weight markers in base pairs. *, P < 0.05; **, P < 0.01; ***, P < 0.001 vs. control (Ctrl).