Figure 7.

Involvement of the MAP kinase pathway in the regulation of caspase-3 activity. (A) Effect of the selective ERK kinase inhibitor U0126 (50 μM) on PACAP-induced inhibition of caspase-3 activity. ***, P < 0.001; NS, not statistically different. (B) Effect of PACAP in the absence or presence of the PKA inhibitor H89 (2 ′ 10⁻⁵ M) and/or the PKC inhibitor chelerythrine (5 ′ 10⁻⁶ M) on ERK phosphorylation 3 or 24 h after plating. (C) Effect of U0126 (50 μM) on PACAP-induced ERK phosphorylation.