Figure 6.

HC-Pro partially suppresses methylation of target DNA. (A) Schematic representation of DNA corresponding to the GUS coding sequence. Positions of HaeIII restriction sites (H1-H5) and sizes (in nucleotides) of the expected digestion products are illustrated. Sites marked by an asterisk contain cytosines in a symmetrical (CpNpG) configuration. Filled circles indicate HaeIII sites that were cytosine methylated in GUS-silenced plants. The right (RB) and left (LB) borders of the GUS transgene are indicated. (B) Blot analysis of genomic DNA in nontransgenic (NT), GUS-silenced transgenic (7 and 17), GUS-nonsilenced transgenic (446), and GUS-silencing suppressed (no. 17HC) plants. Blots were hybridized with a ³²P-labeled probe specific for the GUS gene. The blot was stripped and rehybridized with a ³²P-labeled DNA probe specific for the eIF4E coding sequence.