Table 4.
Colocalization of cytosolic calcium rise and local microfilament accumulation in micropipette-aspirated cells.
| Localization of calcium rise | |||||
|---|---|---|---|---|---|
| Protrusion | Near pipette tip | Elsewhere | No calcium rise | ||
| Localization of microfilament accumulation | Protrusion | 1 | 0 | 0 | 1 |
| Near pipette tip | 1 | 5 | 2 | 3 | |
| Elsewhere | 1 | 0 | 0 | 0 | |
Fourteen individual RBL cells were subjected to micropipette aspiration for 4 minutes under calcium monitoring, then fixed and stained with NBD-phallacidin. All cells displayed local microfilament accumulation, and ten of them exhibited a local calcium rise. The localization of both events is shown.