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. Author manuscript; available in PMC: 2010 Jul 10.
Published in final edited form as: Cell. 2009 Jul 10;138(1):90–103. doi: 10.1016/j.cell.2009.06.021

Figure 2. Effects of TRF1, aphidicolin, and ATRsh.

Figure 2

(A) Examples of the fragile telomeres in TRF1F/F MEF metaphases at day 4 post Cre, Telomeric DNA: FITC PNA probe (green); DNA: DAPI (blue).

(B) Quantification of fragile telomeres induced by deletion of TRF1 with or without treatment with 0.2 µM aphidicolin or ATR shRNA. Bars represent mean values of three independent experiments with SDs. Asterisks: P < 0.01 based on a two-tailed Student's t-test.

(C) Quantification chromosome breaks/gaps. Experimental conditions as in (B).

(D) Quantification of long arms telomere associations in response to the indicated treatments. Experimental conditions as in (B). Sister telomere associations were only scored on long arms. Sister associations were significantly reduced (P < 0.05 based on a two-tailed Student's t-test) by treatment of TRF1 null cells with ATR shRNA but not by aphidicolin treatment or absence of DNA ligase IV (lig4−/−).