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Confocal images of immunofluorescence of KK and KB morula-stage embryos. Anti-KDEL recognizes HSPA5 (detected in green), which is an integral component of ER, and ATF4 is linked to ER stress. Arrow in KB embryo indicates areas in which ER appears to be more prominent than in KK embryos. Red fluorescence (ATF4) also appears more intense in KB embryos (compare merge images and see text). Ab, antibody. Bar = 20 μm.
