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. Author manuscript; available in PMC: 2009 Aug 10.
Published in final edited form as: Mol Microbiol. 2009 Apr 28;72(5):1181–1195. doi: 10.1111/j.1365-2958.2009.06717.x

Fig. 4. GC/MS analysis of volatile organic compounds produced by E. coli transformants expressing sesquiterpene synthases from C. cinereus.

Fig. 4

E. coli cells expressing Cop1 (A) or Cop2 (B) produced as a major compound germacrene A 6a which undergoes Cope rearrangement to β-elemene 6 under the high temperatures of the injection port. E. coli cultures expressing Cop3 (C) accumulated significant quantities of α-muurolene 2, while δ-cadinene 4 was the major product of E. coli cells transformed with Cop4 (D). Peaks are labeled with numbers that correspond to their identified structures shown Fig. 3. Mass spectra for individual peaks are shown in Supplementary Fig. 2. Peak assignments were confirmed by comparing retention indices with that of authentic compounds.