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. 2009 Aug 19;4(8):e6695. doi: 10.1371/journal.pone.0006695

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A, B) Confocal imaging shows anti-CXCL16 (A, left panel) or anti-CXCR6 (A, right panel) or anti-Ki67 (B) as green (488 tyramide), anti-CD3 (594 tyramide) as red, and nuclear staining with Hoechst 33258 as blue. Double staining in yellow shows co-localization. In (A), arrows point to cancer cells and T cells. Panels are representative of more than 15 cases for each double-stain. (C) Staining for Ki67 and CXCR6 in a region of prostatitis using DAB (brown, two left panels) or immunofluorescence (right panel) for detection. Confocal imaging shows anti-CXCR6 as green (488 tyramide) and anti-Ki67 as red (594 tyramide), and nuclear staining with Hoechst 33258 as blue. Panel is representative of more than 15 cases.